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Image Search Results
Journal: The Journal of Pathology
Article Title: YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo‐/radio‐resistance by suppressing caspase‐mediated apoptosis
doi: 10.1002/path.5274
Figure Lengend Snippet: YWHAZ overexpression enhances survival activity to overcome environmental stresses induced by chemo−/radio‐therapy by downregulating caspase‐mediated apoptosis. (A) Western blotting was performed to determine endogenous YWHAZ levels in UCUB cell lines. Levels of β‐actin served as internal controls. (B) RT‐qPCR was performed to determine the relative expression levels of YWHAZ in UCUB cell lines using RT4 cells as the control. (C) Western blotting was performed to determine levels of the indicated effectors involved in caspase‐mediated apoptosis in RT4 cells 48 h after YWHAZ gene transfection. (D) RT4 cells overexpressing YWHAZ were treated with the indicated concentrations of doxorubicin and cisplatin. Cell viability was measured using Alamar Blue assays 4 days after drug treatments. (E) RT4 cells overexpressing YWHAZ were treated with 6 Gy irradiation, after which cell viability was measured every 24 h for 4 days. (F) Incidence rate of preapoptosis among treated cells 48 h after chemo−/radio‐therapy was determined by annexin‐V staining. Cells treated with empty vector served as controls in panels C to F. Data are expressed as means ± SD from five replicates in each experimental group. Paired t ‐tests were performed to evaluate differences between vector‐ and YWHAZ‐treated cells. * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: The BFTC905, HT1376, T24, 5637, TSGH8301, and
Techniques: Over Expression, Activity Assay, Western Blot, Quantitative RT-PCR, Expressing, Control, Transfection, Irradiation, Staining, Plasmid Preparation
Journal: The Journal of Pathology
Article Title: YWHAZ amplification/overexpression defines aggressive bladder cancer and contributes to chemo‐/radio‐resistance by suppressing caspase‐mediated apoptosis
doi: 10.1002/path.5274
Figure Lengend Snippet: YWHAZ overexpression promotes chemo‐resistance. (A) Western blotting was performed to detect YWHAZ levels in RT4 cells showing drug resistance after exposure to various concentrations of doxorubicin or cisplatin. (B) Representative IHC images of paired UCUBs before (primary) and after (recurrent) chemotherapy (left). YWHAZ staining scores were shown in the bar chart using data from 12 paired UCUBs (right).
Article Snippet: The BFTC905, HT1376, T24, 5637, TSGH8301, and
Techniques: Over Expression, Western Blot, Staining